Spark® Cyto
Tecan Spark® Cyto
Plate Reader for 2D & 3D Cell Experiments with Live-Cell Imaging & Real-Time Cytometry
The Spark® Cyto is an advanced live-cell multimode plate reader that combines real-time cytometry, high-speed fluorescence imaging, and full environmental control. It's designed to deliver real-time biological insights for dynamic 2D and 3D cell-based assays.
Technology Overview
High-precision imaging meets automated real-time analysis. Spark Cyto uses full-well bright-field and fluorescence imaging with no stitching, enabling precise tracking of individual cells or entire populations over time.
- Whole-well imaging with 2×, 4×, and 10× objectives
- Autofocus technology ensures consistent image sharpness
- Real-time detection for dynamic biological responses
- Environmental control for temperature, CO₂, O₂, and humidity
Tailored setups for different assay needs and throughput levels. Spark Cyto is highly configurable, allowing labs to choose objectives, plate formats, and detection modes that align with their experimental goals.
- Compatible with 96- and 384-well plates
- Supports absorbance, fluorescence, luminescence, FP, and TRF
- Optional injectors for reagent addition during kinetic assays
- Automation-friendly with plate stackers and readers
Software & Automation
Intuitive software that brings live-cell assays to life. With tools for setup, control, image analysis, and live feedback, Spark Cyto’s software suite reduces complexity while increasing experimental flexibility and reproducibility.
- SparkControl™ for guided workflow setup and plate reading
- Image Analyzer™ for cell segmentation and viability scoring
- REC™ (Real-Time Experimental Control) for live-response automation
- Live Viewer™ for real-time image monitoring
Applications
Built for complex assays across live-cell biology and drug discovery. From cell viability to 3D organoid tracking, Spark Cyto supports a wide range of workflows that demand high sensitivity and reproducibility.
- Cytotoxicity, proliferation, and apoptosis studies
- Spheroid and organoid imaging and analysis
- Wound healing, migration, and cell tracking assays
- Label-free cell counting and viability assessment
Key Benefits
More than a plate reader—Spark Cyto is a live-cell research platform. Combining imaging, detection, and real-time control in one system, Spark Cyto enables faster, more reliable data generation with less manual work.
- Simultaneous multimode readouts and live-cell imaging
- Event-triggered workflows for real-time insights
- Simplified operation, scalable from R&D to screening labs
- Trusted performance with robust traceability
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