Multiplex imaging solutions

PhenoCode™ Panels

PhenoCode™ Panels

Spatial Biology Simplified with DNA Barcoding Technology

PhenoCode™ Panels are pre-validated, ready-to-use multiplex antibody panels that simplify spatial biology workflows. Built for direct integration with PhenoCycler-Fusion 2.0 and PhenoImager HT 2.0, these panels allow researchers to generate rich, reproducible tissue profiling data—fast and accurately, without the complexity of custom validation.

How Does Barcoding Technology Work

Enabling High-Plex Imaging with Precision and Simplicity

Barcoding technology transforms how multiplex tissue imaging is performed. It replaces traditional fluorophores with DNA barcodes to sequentially visualize dozens of markers from a single stain—without damaging the sample.

  • DNA-Barcoded Antibodies: Each antibody is tagged with a unique DNA sequence.
  • Single-Step Staining: All antibodies are applied in a single cocktail to stain the tissue once.
  • Sequential Imaging Cycles: Fluorescent DNA reporters bind barcodes one at a time.
  • Wash, Image, Repeat: The system washes and images each reporter, repeating across markers.
  • High-Plex Output: The complete spatial signature is reconstructed digitally, marker by marker.

Key Benefits

  •  Profile 40–100+ markers without signal overlap
  • Retain tissue for downstream H&E or molecular assays
  • Minimal hands-on time with maximum reproducibility
  • Generate whole-slide, single-cell spatial maps efficiently

Panel Technology Highlights

Pre-Validated Panels for High Performance and Reproducibility

Each PhenoCode panel is developed using stringent validation criteria, ensuring compatibility with a wide range of tissue types and consistent results across runs.

  • Ready-to-use, lyophilized cocktails reduce setup time
  • DNA-barcoded antibodies enable seamless cyclical imaging
  • Validated for FFPE and fresh frozen tissues
  •  <!--[endif]-->High signal retention and minimal background noise

These technical foundations ensure you get high-quality results with less optimization effort.

Seamless Integration

Natively Compatible with PhenoCycler and PhenoImager Platforms

PhenoCode Panels are purpose-built for Akoya's automated imaging platforms—whether for ultrahigh-plex phenotyping or high-speed sample scanning.

  •  <!--[endif]-->PhenoCycler-Fusion 2.0 – Ideal for deep, high-plex spatial biology
  • PhenoImager HT 2.0 – Suited for rapid, region-based imaging across many samples

Whether you’re analyzing entire tissue sections or specific regions of interest, the panels integrate directly into existing workflows—maximizing both efficiency and flexibility.

 Panel Options & Applications

Application-Specific or Customizable Solutions for Every Research Need

PhenoCode Panels are designed to address the most relevant biological and clinical questions in spatial biology. From tumor immunology to tissue structure, each panel offers curated marker sets that save time and deliver robust insight.

Immuno-Oncology Panel

Explore the complexity of the tumor microenvironment (TME).

  • Analyze immune infiltration (e.g., CD8+, CD4+, macrophages)
  • Profile checkpoint molecules like PD-1, PD-L1, CTLA-4
  • Assess spatial relationships between tumor and immune cells

Immunophenotyping Panel

Characterize immune populations and their localization.

  • Detect diverse immune subsets across T, B, NK, dendritic cells
  • Study immune activation, suppression, and migration
  • Useful for autoimmune diseases, infection, and immunotherapy research

Tissue Architecture Panel

Understand tissue structure and its biological context.

  • Map epithelial, stromal, endothelial, and ECM markers
  • Visualize tissue compartments and vasculature
  • Applications in fibrosis, wound healing, cancer histology

Custom Panel Builder

Tailor your own multiplex panel with scientific flexibility.

  • Mix-and-match from Akoya’s validated antibody library
  • Add custom markers relevant to your biology
  • Ideal for translational projects or exploratory biomarker discovery

Benefits of PhenoCode Panels

Efficiency and Reproducibility Built In

PhenoCode Panels are designed to eliminate bottlenecks in assay development while supporting rigorous scientific outcomes.

  • Skip antibody titration and validation entirely
  • Achieve consistent results across samples and operators
  • Accelerate your project from design to discovery
  • Focus on high-impact biology, not setup
  • Enable clinically relevant spatial signatures for diagnostics or therapeutic R&D

 

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