PhenoCycler-Fusion 2.0 Instrument
PhenoCycler-Fusion 2.0, formerly known as CODEX®, is a breakthrough platform for spatial proteomics—offering ultrafast, multiplexed imaging of intact tissues with sample preservation. By combining the scalable automation of PhenoCycler with the high-resolution imaging of PhenoImager (formerly Phenoptics™), researchers can now access comprehensive single-cell data at scale—ideal for both discovery and translational studies.
Scalable Spatial Phenotyping Solutions
From Discovery to Clinical Research
Akoya’s integrated spatial biology ecosystem is designed to support research at every stage—from early discovery to regulated clinical applications. PhenoCycler-Fusion 2.0 sits at the core of this platform, enabling scalable workflows across a range of sample types and study sizes.
- Built for high-throughput biomarker discovery and validation
- Flexible enough to handle exploratory or translational research
- Clinical-ready infrastructure for precision medicine studies
- Seamless integration with AI-powered image analysis tools
PhenoCycler-Fusion Technology Highlights
DNA-barcoded, cyclical imaging without sample damage
This technology uses DNA-tagged antibodies in a one-step staining protocol, followed by iterative binding and imaging cycles. Each round reveals new biomarkers while preserving tissue for further use.
- Antibodies stained once; reporters imaged in cycles
- Enables analysis of over 40–100 markers on a single slide
- Compatible with FFPE, fresh frozen, TMAs, and complex tissues
- Preserves tissue architecture for H&E and post-analysis assays
Instrument Features
Combining speed, flexibility, and precision in one platform
PhenoCycler-Fusion 2.0 is designed for full-slide, high-speed imaging. With support for multiple slides and ultra-fine resolution, it offers flexibility and scale for diverse experimental designs.
- Simultaneous imaging of multiple samples
- Supports high-resolution: 10×, 20×, and 40× magnification
- Full-slide scanning—no need for ROI preselection
- Cycle time as low as 25 minutes for 1.5 × 1.5 cm area
- Integrated automation software for acquisition and QC
- Compatibility with HALO®, Phenochart™, and AI-based analysis
- Phenotype identification, spatial clustering, and neighborhood mapping
- Exportable data for downstream bioinformatics pipelines
Assays & Applications
Multiplexed imaging for a wide range of biological questions
PhenoCycler-Fusion enables researchers to answer complex spatial questions across immune oncology, neurology, fibrosis, infectious disease, and beyond.
- Tumor microenvironment and immune landscape analysis
- Spatial mapping of biomarkers and therapeutic targets
- Tissue heterogeneity and rare cell detection
- Applicable to human and animal tissues, FFPE or frozen
Software & Data Analysis
Turn images into insights with intelligent spatial analytics
The system integrates with advanced software for seamless acquisition, processing, and analysis—providing actionable insights from single cells to tissue-level patterns.
- PhenoCycler software for automated acquisition and QC
- Export to HALO® and Phenochart™ for deep analytics
- Supports phenotype discovery, neighborhood analysis, clustering
- Compatible with AI pipelines for biomarker prediction
Key Performance Advantages
High-content data with unmatched speed and reproducibility Engineered for performance, the system offers high-throughput results without compromising quality—making it ideal for large-scale studies.
